Mfg Part Number
QBD-10184-1000
Vector Labs QBD-10184-1000 dPEG®₈-SATA (S-acetyl-dPEG®₈-NHS ester) - 1000 mg (1/EA)
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dPEG®8-SATA, also known as S-acetyl-dPEG®8-NHS ester, product number QBD-10184, is one of Quanta BioDesign’s versions of the widely popular thiolation reagent N-succinimidyl-S-acetylthioacetate (SATA). The acetyl-protected thiol is separated from the N-hydroxysuccinimidyl (NHS) ester by an amphiphilic, discrete polyethylene glycol (dPEG®) spacer. The dPEG® spacer adds water solubility to the product.
Thiolation is the process of adding a sulfhydryl group to a molecule. Bioconjugation frequently employs thiolation because the reactions to install thiol groups on molecules or to react molecules with sulfhydryl groups are simple and often chemoselective.
The widely popular SATA reagent thiolates molecules with available amines via the NHS ester. Removal of the acetyl protecting group from the sulfhydryl permits crosslinking between the SATA-modified compound and a target molecule of interest that contains a thiol-reactive group, such as maleimide. Unfortunately, SATA is hydrophobic. Consequently, before use, SATA must be dissolved in a dry, water-miscible organic solvent.
Vector Laboratories’ dPEG®8-SATA inserts a single molecular weight octaethylene glycol (dPEG®8) spacer between the protected thiol and the NHS ester. The dPEG® spacer imparts water solubility to the molecule, allowing dPEG®8-SATA to dissolve and react in water without needing an organic solvent. Also, the dPEG®8 spacer adds hydrodynamic volume to the molecule to which it is conjugated. Increasing the hydrodynamic volume reduces aggregation and precipitation of proteins conjugated to dPEG®8-SATA. Hydroxylamine hydrochloride (CAS number 5470-11-1) easily removes the acetyl protecting group, exposing the sulfhydryl moiety for further reaction. Any application that can be carried out with a traditional, non-PEGylated SATA reagent can be carried out with our SATA containing a dPEG® spacer.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1000 mg |