Mfg Part Number
QBD-10202-1000
Vector Labs QBD-10202-1000 NHS-dPEG®₄-biotinidase resistant biotin - 1000 mg (1/EA)
Quick Overview
TFP-dPEG₄-biotinidase resistant biotin - 100 mg
$1,449.66
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NHS-dPEG®4-biotinidase-resistant biotin, product number QBD-10202, is a water-soluble biotinylation reagent modified to resist hydrolysis by biotinidase (EC 3.5.1.12). This product combines Biotinoyl-2-aminobutyric acid with the N-hydroxysuccinimidyl (NHS) ester of a single molecular weight, discrete polyethylene glycol (dPEG®) linker. NHS-dPEG®4-biotinidase-resistant biotin retains biotin’s typical high affinity for avidin and streptavidin. However, the modified biotin prevents recycling by biotinidase. This product is useful in applications such as ELISAs of tissue samples and antibody pretargeting, where endogenous biotinidase can recycle biotin.
Biotinidase is an amidohydrolase that breaks the covalent bond between biotin and amino acids such as lysine (i.e., biocytin). In vivo, biotinidase recycles biotin. Although necessary for healthy bodily function, biotinidase potentially creates problems when biotinylated proteins are used in serological assays, since endogenous biotinidase removes the biotin from these proteins. Dr. D. Scott Wilbur and colleagues at the University of Washington in Seattle developed biotinidase-resistant biotin in 2006 to facilitate in vivo antibody pretargeting. However, to improve the product’s water solubility, a short amino-dPEG®4-propionic acid linker was conjugated to the biotinidase-resistant biotin and subsequently functionalized as an NHS ester. Like our other dPEG® biotinylation products, NHS-dPEG®4-biotinidase-resistant biotin will not cause aggregation and precipitation of biomolecules to which it is conjugated, and the dPEG® spacer reduces non-specific binding. NHS-dPEG®4-biotinidase-resistant biotin resists amide hydrolysis because of the non-natural amino acid to which it is conjugated. It is potentially beneficial for applications using serological fluids that contain endogenous biotinidase. Useful roles for NHS-dPEG®4-biotinidase-resistant biotin include the following:
therapeutic applications such as antibody pretargeting with biotin-streptavidin systems;
supramolecular constructs (e.g., nanoparticles) used in vivo that depend on biotin-(strept)avidin affinity to maintain structural integrity; and,
ELISAs of serological fluids and tissue samples using biotin-(strept)avidin affinity.
Biotinidase is an amidohydrolase that breaks the covalent bond between biotin and amino acids such as lysine (i.e., biocytin). In vivo, biotinidase recycles biotin. Although necessary for healthy bodily function, biotinidase potentially creates problems when biotinylated proteins are used in serological assays, since endogenous biotinidase removes the biotin from these proteins. Dr. D. Scott Wilbur and colleagues at the University of Washington in Seattle developed biotinidase-resistant biotin in 2006 to facilitate in vivo antibody pretargeting. However, to improve the product’s water solubility, a short amino-dPEG®4-propionic acid linker was conjugated to the biotinidase-resistant biotin and subsequently functionalized as an NHS ester. Like our other dPEG® biotinylation products, NHS-dPEG®4-biotinidase-resistant biotin will not cause aggregation and precipitation of biomolecules to which it is conjugated, and the dPEG® spacer reduces non-specific binding. NHS-dPEG®4-biotinidase-resistant biotin resists amide hydrolysis because of the non-natural amino acid to which it is conjugated. It is potentially beneficial for applications using serological fluids that contain endogenous biotinidase. Useful roles for NHS-dPEG®4-biotinidase-resistant biotin include the following:
therapeutic applications such as antibody pretargeting with biotin-streptavidin systems;
supramolecular constructs (e.g., nanoparticles) used in vivo that depend on biotin-(strept)avidin affinity to maintain structural integrity; and,
ELISAs of serological fluids and tissue samples using biotin-(strept)avidin affinity.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1000 mg |