Mfg Part Number
QBD-10374-1000
Vector Labs QBD-10374-1000 SPDP-dPEG®₄-NHS ester - 1000 mg (1/EA)
Quick Overview
SPDP-dPEG₈-NHS ester - 100 mg
$1,300.48
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SPDP-dPEG®4-NHS ester, product number QBD-10374, is Vector Laboratories’ water-soluble version of the immensely popular, but unfortunately hydrophobic, SPDP crosslinker. SPDP-dPEG®4-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is a short (20 atoms), amphiphilic, single molecular weight, discrete polyethylene glycol (dPEG®) spacer.
Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.
By contrast, SPDP-dPEG®4-NHS ester, product number QBD-10374, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1000 mg |