Details
The 10 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge or a vacuum manifold capable of running 96-well plates. IBI offers IB47500 96-well vacuum manifold.
Typical yields are 20 – 35 µg for high-copy number plasmid and 3 – 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions.
Features:
• 10 x 96 Well Plasmid Kit
• Centrifuge or vacuum format
• 1 – 5 mL cultured bacterial cells
• Filter plates, deep well plates, and collection plates included