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Eurogentec Takyon™ One-Step ROX Probe 5X Mastermix, 5 x 7.5 ml

Vendor: Eurogentec

Mfg Part Number: UF-RP5X-RT0505

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Quick Overview

RNA Target
Probe-Based Detection
ROX Included
6250 rxn

Protocol: PDF

MSDS: PDF

Price: $4871.31

In stock items typically ship within 24 hours

Quick Overview

RNA Target
Probe-Based Detection
ROX Included
6250 rxn

Details

Takyon™ One-Step ROX Probe 5X MasterMix dTTP is a convenient, optimized 5X concentrated mixture of premeasured ingredients in a ready-to-use format for the performance of one-step qRT-PCR from total or purified mRNA samples using fluorescent, gene-specific probe molecules. Obtain consistent and sensitive results under Fast or regular cycling conditions. In a single pipetting step, add EuroScript II MMLV Reverse Transcriptase and RNase inhibitor to MasterMix formulated with a chemically modified hot-start 3rd generation Phusion DNA polymerase (activated in just three minutes at 95°C). 5X concentrated reaction buffer offers the advantage of more dead volume for DNA template. Incorporates ROX passive reference dye to permit sample fluorescence normalization. Refer to Technical Data Sheet for kit components and cycling conditions. Compatible with ABI Prism® 7000, 7300, 7700 & 7900 and ABI StepOne™ & StepOnePlus™.

Additional Information

Applications 1-Step qRT-PCR
RT System Compatibility N/A
Detection Chemistry Probe
dUTP No
Fast Protocol Compatible Yes
Passive Reference Dye ROX
Hot Start Yes
Concentration 5X
Multiplex Capable Yes
Number of Reactions 6250
Kit Volume 5 x 7.5 ml
Reaction Volume (µL) 20 µL
Target RNA
UNG Included No


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