Details
Forget-Me-Not™ qPCR Master Mix is a hot-start EvaGreen dye-based master mix for use in real time PCR applications and DNA Melting Curve Analysis. Forget-Me-Not™ features a unique combination of a master mix containing a low concentration of blue dye, plus a DNA template buffer containing a higher concentration of blue dye. When you add the 2X Forget-Me-Not™ Master Mix to your reaction, it appears light blue. Then, when you add template containing Forget-Me-Not™ Template Buffer to the reaction, the color turns dark blue. Forget-Me-Not™ allows you to see at a glance whether you forgot to add master mix or template to any of your reactions, so you can catch pipetting mistakes and avoid wasting time, reagents, and your precious DNA samples. The 2X Forget-Me-Not™ Master Mix also can be used without the Template Buffer if you prefer.
Includes separate vial of ROX.