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Reagents

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  1. OneStep qMethyl™ '**'  ^
  2. 5X MN Stop Buffer (6 ml)
  3. MN Digestion Buffer (50 ml)
  4. Nuclei Prep Buffer (30 ml)
  5. Nuclei Prep Buffer (50 ml)
  6. Micrococcal Nuclease (10 U /100 μl) ^
  7. EZ Nucleosomal DNA Prep Kit (20 Preps)
  8. Zymo Research D5210-7-1 5M NaCl
  9. 5X Chromatin Elution Buffer 10 ml
  10. Chromatin Wash Buffer III 30 ml
  11. Chromatin Wash Buffer II 30 ml
  12. Chromatin Wash Buffer I 30 ml
  13. Chromatin Dilution Buffer 30 ml
  14. Chromatin Shearing Buffer 30 ml
  15. Zymo-Spin™ ChIP Kit (25 preps) Mechanical Shearing Protocol '*'

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