IBI Scientific Isolate Total Extraction Reagent Kit (200 mL)

IBI Isolate is a phenol, chloroform, and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA, and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells, and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA library construction, cloning, RT-PCR (Endpoint), real-time PCR, nuclease protection assays, and northern blotting.

IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Features:
• Sample Size: Scalable for blood, buffy coat, serum, plasma cultured cells and tissue
• Operation time: up to 60 minutes
• Can simultaneously extract DNA – RNA and Protein from the same sample.