IBI Scientific Large Fragment DNA Gel Extraction Kit (100 Prep)

The Large DNA Fragment Extraction Kit was designed to recover or concentrate a broad range of DNA fragments (100bp – 50Kb) from agarose gel, PCR, or other enzymatic reactions. A concentrated sodium buffer is used to dissolve agarose gel and denature enzymes. DNA fragments are then bound by silica beads. Contaminants are removed with Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE Buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. 10 – 20µl of Elution Buffer is used to obtain a high concentration of purified DNA which is ready for use in downstream applications.

The Large DNA Fragment Extraction Kit includes pH indicator for easy determination of optimal pH and sodium acetate to adjust pH if it becomes too high following gel dissociation or PCR product reaction.

The quality of the Large DNA Fragments Extraction Kit is tested on a lot-to-lot basis by purifying DNA fragments from agarose gel, PCR products, or other aqueous solutions and analyzed by electrophoresis.

Features:
• Sample Size: up to 250mg agarose gel/up to 100µl PCR products
• Fragment Size: 100bp – 50Kb
• Binding Capacity: 5µg DNA per 10µl of silica beads
• Expectant Yield: 100bp – 10Kb=70-95%, >10Kb=60-80%
• Operation Time: 20 min for PCR Cleanup/30 min for Gel extraction