Quick Overview
Ethidium Bromide Stain
10 mL of 10 mg/mL solution in Water
10 mL of 10 mg/mL solution in Water
Details
Ethidium bromide (EB) is a widely used nucleic acid gel stain although it is also a known potent mutagen. EB is an intercalating DNA-binding dye with little sequence preference. Once bound to nucleic acids, the fluorescence of the dye is enhanced by more than 10 times while the excitation maximum is red shifted by 30-40 nm and emission is blue-shifted by about 15 nm. Because of its toxicity, Biotium offers EB solution in H2O as a safer alternative to weighing out the powdery form.
- λEx/λEm (with DNA) = 518/605 nm
- Red solution
- Store at 4°C and protect from light
- C21H20BrN3
- Mwt: 394.31
- λEx/λEm (with DNA) = 518/605 nm
- Red solution
- Store at 4°C and protect from light
- C21H20BrN3
- Mwt: 394.31
Additional Information
| Concentration | N/A |
|---|---|
| Gel Compatibility | Agarose, Polyacrylamide |
| Gel Stain | Ethidium Bromide |
| Nucleic Acid | dsDNA, RNA, ssDNA |
| Quantity | 10 |
| Tracking Dye Color | N/A |
