IBI Scientific Mini Genomic DNA Kit-Blood/CC Kit (100 Prep)

The Genomic DNA Mini Kits for Blood and Cultured Cells provide fast and economical method for the purification of total DNA (including genomic, mitochondrial, and viral DNA) from fresh whole blood, plasma, serum, buffy coat, other bodily fluids, lymphocytes bacteria, and cultured cells. The method uses a chaotropic salt to lyse cells and degrade proteins. The DNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. After removal of contaminants, the purified DNA is eluted by a low salt elution buffer or water. The entire procedure can be completed in 40 minutes without the use of phenol extraction or alcohol precipitation.

The quality of the Genomic DNA Mini Kits for Blood and Cultured Cells is tested on a lot-to-lot basis by isolating genomic DNA from 200 µl of whole human blood. The purified DNA (4-6 µg with an A260/A280 ratio of 1.6 – 1.8) is quantified with a spectrophotometer and checked by electrophoresis.

Features:
• Sample Size: up to 300µl fresh whole blood/up to 107 animal cultured cells
• Expectant Yield: up to 50µg DNA
• Operation Time: 40 min or less
• Binding Capacity: 10µg per well
• Elution Volume: 50 – 200µl