IBI Scientific Mini Hi-Speed Plasmid Kit (300 Prep)

The Hi Speed Mini Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-5ml of bacterial cultures. The modified alkaline lysis method and RNase treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate, binds to the glass-fiber matrix in the spin column. The contaminants are washed off with an ethanol based wash buffer. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol plasmid. Typical yields are 20-30 µg for high-copy numbered plasmids or 3-10 µg for low-copy numbered plasmids. The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, or sequencing reactions. The entire procedure can be completed in under 30 minutes.

The quality of the Hi Speed Plasmid Mini Kit is tested on a lot-to-lot basis, by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600>2U/ml). Following the purification process, a yield of more than 20 µg is expected and the ratio of A260/A280 is between 1.7-1.9. The purified plasmid (1 µg) is used in EcoRI digestion and checked by electrophoresis

Features:
• Sample Size: 1 – 4 mL bacterial culture
• Expectant Yield: 20-30 µg for high-copy plasmids/3-10 µg for low-copy plasmid
• Operation Time: 30 min or less
• Elution Volume: 50-100 µL
• Binding Capacity: up to 30 µg