Quick Overview
Enzyme and digestion buffer used for cutting both double stranded and single-stranded DNA.
Details
Zymo Research DNase I is a recombinant endonuclease that cleaves single- and double-stranded DNA. It digests DNA more efficiently than other DNase I, making it optimal for sensitive applications like NGS (RNA-seq) and PCR. It is extensively purified, and quality controlled to ensure RNase-free and protease-free.
Zymo Research DNase I is lyophilized and supplied with DNA digestion buffer. It dissolves in water within seconds and remains stable for up to 10 freeze-thaw cycles. Customized, OEM, and bulk orders are available.
Technical Specifications:
Unit Definition - One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
Storage - After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
Reconstitution - Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
Inactivation - Heat inactivate at 75 °C for 10 min with 5 mM EDTA.
Zymo Research DNase I is lyophilized and supplied with DNA digestion buffer. It dissolves in water within seconds and remains stable for up to 10 freeze-thaw cycles. Customized, OEM, and bulk orders are available.
Technical Specifications:
Unit Definition - One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
Storage - After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
Reconstitution - Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
Inactivation - Heat inactivate at 75 °C for 10 min with 5 mM EDTA.
Additional Information
| Brand | Zymo Research |
|---|---|
| UNSPSC | 41120000 |
| Product Application | DNA degradation |
| Default Product Type | No |
| Type Of Product | Protein and Enzymes |
| Manufacturer | No |
