Sartorius VN02H21 VivaconŽ 2. 30.000 MWCO Hydrosart. 25pc

pVivaconŽ 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, VivaconŽ 2 is equipped with patented HydrosartŽ regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg./p pHigh recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. VivaconŽ 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASPsup1/sup). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations./p psup1/supWisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62./p ulliConcentration of up to 2 ml genomic DNA or peptide samples/liliLow binding material/liliReverse spin possibility/liliMWCO printed on device/liliComplete and highly reproducible concentrate recovery/liliSelected cut offs available sterile and DNA free* for e.g. forensic applications/lili* qPCR assay over 55 cycles with a verified limit of detection 2 pg/li /ul h3Performance Characteristics for DNA/h3 pStart volume 2 ml, sample concentration 50 ng/ml./p!-- Table - Vivacon 2 -- tabletheadtrth scope="col"/thth scope="col"Sample size (bp)/thth scope="col"Time to concentrate upbr /to 30x [min.] at 20°C/thth scope="col"Concentratebr /recovery %/thth scope="col"g-force (xg)/th/tr/theadtbodytrtd2,000 MWCO/tdtd10/tdtd120 min./tdtd92 %/tdtd7,500/td/trtrtd10,000 MWCO/tdtd30/tdtd60 min./tdtd94 %/tdtd5,000/td/trtrtd30,000 MWCO/tdtd50/tdtd60 min./tdtd95 %/tdtd2,500/td/trtrtd50,000 MWCO/tdtd300/tdtd45 min./tdtd96 %/tdtd2,500/td/trtrtd100,000 MWCO/tdtd600/tdtd30 min./tdtd93 %/tdtd2,500/td/trtrtd125,000 MWCO/tdtd650/tdtd30 min./tdtd88 %/tdtd2,500/td/trtrtd125,000 MWCO/tdtd900/tdtd30 min./tdtd89 %/tdtd2,500/td/tr/tbody/tableh3Performance Characteristics for Proteins/h3Start volume 2 ml, sample and concentration of proteins as specified in table.!-- Table 2 - Vivacon 2 -- table thead tr th scope="col" /th th scope="col" Sample/th th scope="col" Time to concentrate upbr / to 30x [min.] at 20°C/th th scope="col" Concentratebr / recovery %/th th scope="col" g-force (xg)/th /tr /thead tbody tr td 2,000 MWCO/td td 0.25 mg/mlbr / cytochrome c/td td 120 min./td td 95 %/td td 7,500/td /tr tr td 10,000 MWCO/td td 0.25 mg/mlbr / cytochrome c/td td 90 min./td td 96 %/td td 5,000/td /tr tr td 30,000 MWCO/td td 1.0 mg/ml BSA/td td 40 min./td td 96 %/td td 5,000/td /tr tr td 50,000 MWCO/td td 1.0 mg/ml BSA/td td 30 min./td td 94 %/td td 5,000/td /tr tr td 100,000 MWCO/td td 1.0 mg/mlbr / bovine IgG/td td 30 min./td td 92 %/td td 5,000/td /tr tr td 125,000 MWCO/td td 1.0 mg/mlbr / bovine IgG/td td 27 min./td td 81 %/td td 5,000/td /tr /tbody/tableStart volume 2 ml, sample and concentration of proteins as specified in table.!-- Table 2 - Vivacon 2 -- table thead tr th scope="col" /th th scope="col" Sample/th th scope="col" Time to concentrate upbr / to 30x [min.] at 20°C/th th scope="col" Concentratebr / recovery %/th th scope="col" g-force (xg)/th /tr /thead tbody tr td 2,000 MWCO/td td 0.25 mg/mlbr / cytochrome c/td td 120 min./td td 95 %/td td 7,500/td /tr tr td 10,000 MWCO/td td 0.25 mg/mlbr / cytochrome c/td td 90 min./td td 96 %/td td 5,000/td /tr tr td 30,000 MWCO/td td 1.0 mg/ml BSA/td td 40 min./td td 96 %/td td 5,000/td /tr tr td 50,000 MWCO/td td 1.0 mg/ml BSA/td td 30 min./td td 94 %/td td 5,000/td /tr tr td 100,000 MWCO/td td 1.0 mg/mlbr / bovine IgG/td td 30 min./td td 92 %/td td 5,000/td /tr tr td 125,000 MWCO/td td 1.0 mg/mlbr / bovine IgG/td td 27 min./td td 81 %/td td 5,000/td /tr /tbody/table