Details
pVivaspinŽ concentrators are disposable ultrafiltration devices for the concentration of biological samples. VivaspinŽ 2 can effectively be used in swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations, even with particle-laden solutions. VivaspinŽ 2 combines the speed of the classic VivaspinŽ products with low internal surface and membrane area in order to achieve superior recoveries from very dilute solutions. Available with a choice of PES, Cellulose Triacetate or HydrosartŽ membranes, VivaspinŽ 2 offers the highest flexibility for process optimization. Another unique feature of VivaspinŽ 2 is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator or alternatively reverse-spinning the concentrate into the recovery cap. Both methods result in near total concentrate recovery./p ulliChoice of three different membranes/liliHigh retentate recovery 90 %/liliVertical membrane design for ultra-fast concentration/liliUniversal rotor compatibility/liliSafe sample concentration due to dead-stop volume/liliConvenient concentrate recovery by pipetting/liliOptional re-spin for concentrate recovery/liliVolume graduation for easy reading/monitoring/li /ul h3Performance Characteristics/h3 tabletheadtr th scope="col"/th th scope="col" Time to concentrate up to 30x [min.] at 20°C/th th scope="col" Concentrate recovery %/th/tr/theadtbodytr td Start volume/td td 500 ?l/td td 500 ?l/td/trtr td colspan="3" Aprotinin 0.25 mg/ml (6,500 MW)/td/trtr td 3,000 MWCO PES/td td 30/td td 96 %/td/trtr td colspan="3" BSA 1.0 mg/ml (66,000 MW)/td/trtr td 5,000 MWCO PES/td td 15/td td 96 %/td/trtr td 10,000 MWCO PES/td td 5/td td 96 %/td/trtr td 30,000 MWCO PES/td td 5/td td 95 %/td/trtr td colspan="3" IgG 0.25 mg/ml (160,000 MW)/td/trtr td 30,000 MWCO PES/td td 10/td td 96 %/td/trtr td 50,000 MWCO PES/td td 10/td td 96 %/td/trtr td 100,000 MWCO PES/td td 10/td td 96 %/td/tr/tbody /table