Details
pVivaspinŽ 15R has all the unique features of the Sartorius centrifugal concentrators, including a patented vertical twin membrane and a dead stop. VivaspinŽ 15R is targeting the volume segment of 2 to 15 ml with the HydrosartŽ regenerated cellulose membrane. This membrane is ideal when extremely high recovery rate and very low adsorption is required, for example in applications such as concentration and desalting of immunoglobulin fractions./p ulliHighest recovery at low absorption (95-98%)/liliExtremely short concentration time (30-fold in 15 min.)/liliVery small holdup volume ( 20 l)/liliCrystal clear housing and volume graduations/liliVertical twin membrane for ultra-fast concentration/liliSafe sample concentration due to dead-stop volume/li /ul h3Rotor Compatibility/h3 pPlease note: VivaspinŽ 15R (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the VivaspinŽ Turbo 15, which can be used in either round bottom or conical centrifuge tubes./p h3Performance Characteristics/h3 !-- Table - VivaspinŽ 15r -- tabletheadtr th scope="col" /th th colspan="4" rowspan="1" scope="col" Time to concentrate up to 30x [min.] atbr / 20°C and solute recovery %/th/tr/theadtbodytr td Rotor/td td colspan="2" Swing Bucket/td td colspan="2" rowspan="1" 25° Fixed angle/td/trtr td Start volume/td td colspan="2" 15 ml/td td colspan="2" rowspan="1" 12.5 ml/td/trtr td /td td Min./td td Rec./td td Min./td td Rec./td/trtr td colspan="5" Insulin chain A 0.1 mg/ml* (2,500 MW)/td/trtr td 2,000 MWCO/td td 70/td td 96 %/td td 60/td td 96 %/td/trtr td colspan="5" Aprotinin 0.1 mg/ml* (6,500 MW)/td/trtr td 5,000 MWCO/td td 47/td td 95 %/td td 45/td td 95 %/td/trtr td colspan="5" Cytochrome c 0.25 mg/ml* (12,400 MW)/td/trtr td 5,000 MWCO/td td 45/td td 96 %/td td 45/td td 96 %/td/trtr td 10,000 MWCO/td td 25/td td 94 %/td td 18/td td 94 %/td/trtr td colspan="5" a-chymotrypsin 0.25 mg/ml* (25,000 MW)/td/trtr td 5,000 MWCO/td td 50/td td 98 %/td td 45/td td 98 %/td/trtr td 10,000 MWCO/td td 25/td td 98 %/td td 18/td td 98 %/td/trtr td colspan="5" Ovalbumin 1.0 mg/ml* (45,000 MW)/td/trtr td 10,000 MWCO/td td 20/td td 98 %/td td 14/td td 98 %/td/trtr td 30,000 MWCO/td td 15/td td 94 %/td td 12/td td 94 %/td/trtr td colspan="5" BSA 1.0 mg/ml* (66,000 MW)/td/trtr td 30,000 MWCO/td td 18/td td 98 %/td td 15/td td 98 %/td/trtr td colspan="5" IgG 0.1 mg/ml* in DMEM (160,000 MW)/td/trtr td 30,000 MWCO/td td 30/td td 98 %/td td 25/td td 96 %/td/tr/tbody /table pproteins other than IgG made up in 50 mM potassium sulphate, 150 mM sodium chloride, pH 7.4/p