Sartorius VS2061 VivaspinŽ 20. 1.000.000 MWCO PES. 12pc

pVivaspinŽ 20 centrifugal concentrators offer increased volume flexibility and performance to the VivaspinŽ potfolio. VivaspinŽ 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. VivaspinŽ 20 features twin vertical membranes for unparalleled filtration speed and 100-fold concentration. Volume graduation allows monitoring the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, VivaspinŽ 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is can be placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, VivaspinŽ 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. 'Pressure-fugation' is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential./p ulliHigh flux PES membrane/liliHigh retentate recovery 90 %/liliVertical twin membrane for ultra-fast concentration/liliUniversal rotor compatibility/liliSafe sample concentration due to dead-stop volume/liliConvenient concentrate recovery by pipetting/liliVolume graduation for easy reading/monitoring/lili100-fold concentration with twin vertical membranes/liliAdditional option for pressurized operation/li /ul h3Rotor Compatibility/h3 pPlease note: VivaspinŽ 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the VivaspinŽ Turbo 15, which can be used in either round bottom or conical centrifuge tubes/p h3Performance Characteristics/h3 !-- Table - VivaspinŽ 20 -- tabletheadtr th scope="col"/th th colspan="4" rowspan="1" scope="col" Time to concentrate up to 30x [min.] at 20°Cbr / and solute recovery %/th/tr/theadtbodytr td Mode/td td Centrifuge/td td Centrifuge/td td Bench top/td td Press-fuge/td/trtr td Rotor/td td Swing bucket/td td 25° Fixed angle/td td Pressure/td td Swing bucket/td/trtr td Sart volume/td td 20 ml/td td 14 ml/td td 10 ml/td td 10 ml/td/trtr td/td td Min./Rec./td td Min./Rec./td td Min./Rec./td td Min./Rec./td/trtr td colspan="5" Cytochrome c 0.25 mg/ml (12,400 MW)/td/trtr td 3,000 MWCO PES/td td 110 / 97 %/td td 180 / 96 %/td td 60 / 96 %/td td - / -/td/trtr td colspan="5" BSA 1.0 mg/ml (66,000 MW)/td/trtr td 5,000 MWCO PES/td td 23 / 99 %/td td 29 / 99 %/td td 50 / 98 %/td td 14 / 98 %/td/trtr td 10,000 MWCO PES/td td 16 / 98 %/td td 17 / 98 %/td td 32 / 97 %/td td 8 / 97 %/td/trtr td 30,000 MWCO PES/td td 13 / 98 %/td td 15 / 98 %/td td 32 / 97 %/td td 8 / 97 %/td/trtr td colspan="5" IgG 0.25 mg/ml (160,000 MW)/td/trtr td 30,000 MWCO PES/td td 27 / 97 %/td td 20 / 95 %/td td 46 / 94 %/td td 13 / 97 %/td/trtr td 50,000 MWCO PES/td td 27 / 96 %/td td 22 / 95 %/td td 46 / 93 %/td td 13 / 96 %/td/trtr td 100,000 MWCO PES/td td 25 / 91 %/td td 20 / 90 %/td td 42 / 88 %/td td 12 / 94 %/td/trtr td colspan="5" Latex beads 0.004% in DMEM 10% FCS (0.055 ?m)/td/trtr td 300,000 MWCO PES/td td 20 / 99 %/td td 35 / 99 %/td td 10 / 99 %/td td - / -/td/trtr td Latex beads 0.004% in DMEM 10% FCS (0.24 ?m)/td td/td td/td td/td td/td/trtr td 1,000,000 MWCO PES/td td 4 / 99 %/td td 12 / 99 %/td td 4 / 99 %/td td - / -/td/trtr td colspan="5" Yeast 1.0 mg/ml (S. Cerevisiae)/td/trtr td 0.2 ?m PES/td td 15 / 95 %/td td 5 / 95 %/td td 20 / 95 %/td td 2 / 95 %/td/tr/tbody /table