September 13, 2024

Overcoming challenges with DPBS calcification and how to avoid it during your shipping and storage process post purchase

What is DPBS?

Dulbecco's Phosphate-Buffered Saline (DPBS) is a commonly used buffer solution in biological and biochemical research. It often contains salts, including calcium and magnesium, which are important for maintaining cellular functions and osmotic balance. However, under certain conditions, DPBS can experience calcification (precipitation of calcium salts).

When storing, it is important to keep all DPBS solutions in a cool place away from direct sunlight. Increased temperature can increase the rate of reactions and may impact solubility, often leading to precipitation under certain circumstances.

Prolonged storage of DPBS, especially at room temperature, may lead to precipitation as the system reaches an equilibrium where these insoluble salts begin to form. High concentrations of calcium (Ca²⁺) and phosphate (PO₄^3-) can lead to supersaturation and subsequently precipitation of calcium phosphate compounds, such as hydroxyapatite, which can form deposits. If the solution is left open, evaporation of water can concentrate the remaining ions, increasing the likelihood of precipitation.

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Master shipping and storing DPBS

It's important to control these variables when working with DPBS to minimize or prevent calcification, especially in applications that require the integrity of the solution to maintain cell viability or support biochemical applications.

Select appropriate containers that are leak-proof and can withstand handling. In addition to the original packaging, an outer box or cooler that holds the primary container can ensure that it's sturdy and insulated if necessary to avoid any environmental impacts (such as hot summers in Texas)!

If appropriate, consider using gel packs or dry ice if thermal stability is a concern during transit, especially for long-distance shipping.

We always suggest utilizing a shipping carrier experienced in handling biological materials to ensure proper handling and delivery. We suggest FedEx or UPS shipping services.

Do not store DPBS for long periods of time. It is recommended to store it unopened at room temperature for about 6 to 12 months. Once opened, it is recommended to store it in the refrigerator at 2-8°C and use it within 1 month to avoid contamination.

When freezing DPBS, which can extend its shelf life significantly, ensure it is aliquoted to avoid repeated freeze-thaw cycles, which can degrade the solution's effectiveness. Frozen DPBS can often be stored for several months to a year, but it’s always best to consult specific guidelines for your particular application.