Mfg Part Number
CCT-1296-1
Vector Labs CCT-1296-1 AZDye 594 Picolyl Azide - 1 mg (1/EA)
Quick Overview
AZDye 594 Picolyl Azide - 1 mg
$231.95
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AZDye™ 594 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye structurally is identical to Alexa Fluor® 594. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF® 594 Dye, DyLight® 594 and Alexa Fluor® 594.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161506 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1 mg |