Mfg Part Number
QBD-10201-50
Vector Labs QBD-10201-50 Biotin-dPEG®₃-MAL - 50 mg (1/EA)
Quick Overview
NHS-dPEG₄-biotinidase resistant biotin - 100 mg
$170.07
Order will ship within 1-3 business days
Biotin-dPEG®3-MAL, product number 10201, is one of three products containing biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other. For QBD-10201, the linker is a dPEG®3 product. The flexible, short-chain, discrete PEG (dPEG®) linker imparts water solubility to conjugates formed with it. The maleimide moiety is a popular reactive group for conjugating to free thiol groups in biomolecules and other sulfhydryl compounds. Maleimide groups can be conjugated to free thiol groups in the pH range 6.5 – 7.5, forming a stable thioether linkage.
This product has proven helpful in various applications that utilize the high streptavidin-biotin binding affinity. Such applications include
FRET-based assays
probes of protein topology
protein labeling
the creation of biotinylated, dPEG®-modified nucleosides
different types of high-throughput screening procedures
the construction of biomimetic gold-and-silica nanoparticles, and
the construction of self-assembling quantum dots. To use Biotin-dPEG®3-MAL, dissolve the product in a dry solvent and add it to an aqueous compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent for this purpose. For biomolecules (e.g., antibodies), care should be taken to use a minimal amount of organic solvent so as not to denature the biomolecule or cause salt precipitation.
FRET-based assays
probes of protein topology
protein labeling
the creation of biotinylated, dPEG®-modified nucleosides
different types of high-throughput screening procedures
the construction of biomimetic gold-and-silica nanoparticles, and
the construction of self-assembling quantum dots. To use Biotin-dPEG®3-MAL, dissolve the product in a dry solvent and add it to an aqueous compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent for this purpose. For biomolecules (e.g., antibodies), care should be taken to use a minimal amount of organic solvent so as not to denature the biomolecule or cause salt precipitation.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 50 mg |