Mfg Part Number
CCT-1107-25
Vector Labs CCT-1107-25 Desthiobiotin Azide - 25 mg (1/EA)
Quick Overview
Desthiobiotin Azide - 25 mg
$517.04
Order will ship within 1-3 business days
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161506 |
| Reagent Application | Bioconjugation |
| Reagent Size | 25 mg |