Mfg Part Number
CCT-1308-5
Vector Labs CCT-1308-5 AZDye 405 Picolyl Azide - 5 mg (1/EA)
Quick Overview
AZDye 405 Picolyl Azide - 5 mg
$720.36
Order will ship within 1-3 business days
AZDye™ 405 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
AZDye™ 405 is structurally similar and spectrally is almost identical to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, or any other sulfonated pyrene based fluorescent dyes.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161506 |
| Reagent Application | Bioconjugation |
| Reagent Size | 5 mg |