Mfg Part Number
FP-1731-5
Vector Labs FP-1731-5 UBQ-1 NHS Ester - 5 mg (1/EA)
Quick Overview
UBQ-2 NHS Ester - 100 mg
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Universal Black 1 Quencher (UBQ-1, Black Hole Quencher®-2 (BHQ®-1) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 480-580 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with green to yellow emitting dyes such as FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 5 mg |