Enzo ALX-201-059-U025 Caspase-3 (human), (recombinant) (active) (25 U)

CPP32, Yama, Apopain

Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-216 or ALX-850-215).

Lyophilized.

Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-pNA (Prod. No. ALX-260-033) and Ac-DEVD-AFC (Prod. No. ALX-260-032).

Reconstitute to 1U/µl in water.

Produced in E. coli. Contains an N-terminal His-tag.

≥15,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (Prod. No. ALX-260-033) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.

P42574

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

-80°C

Dry Ice