Mfg Part Number
ALX-201-062-U100

Enzo ALX-201-062-U100 Caspase-8 (human), (recombinant) (active) (100 U)

Quick Overview
Enzo ALX-201-062-U100 Caspase-8 (human), (recombinant) (active) (100 U)
$699.92
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Alternative Name

FLICE, Mch5, MACH

Application Notes

Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.

Formulation

Lyophilized.

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute to 1U/µl with PBS containing 15% glycerol.

Source

Produced in E. coli. Contains an N-terminal His-tag.

Specific Activity

5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.

UniProt ID

Q14790

Handling

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Long Term Storage

-80°C

Shipping

Dry Ice

More Information
Brand Enzo
UNSPSC 12352202