Mfg Part Number
40022
Biotium Biotin-16-dUTP, 1 mM solution
Quick Overview
Biotin-16-dUTP
1 mM Solution
1x 50 µL
$367.16
Order will ship within 1-3 business days
Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′ end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number ’16’ is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-11-dUTP (catalog no. 40029) and biotin-20-dUTP (catalog no. 40030). The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin.
- 1 mM solution in pH 7.5 Tris-HCl buffer
- Store at -20°C
- C32H48N7O18P3Sli4
- MW: 971.5
| UNSPSC | 12352200 |
|---|---|
| Buffer | Tris-HCL |
| Concentration | 1 mM |
| Label | Biotin |
| Nucleotides | dUTP |
| Quantity | 50 |