Enzo ADI-905-630-100 Osteopontin monoclonal antibody (87-B) (100 µg)
| Alternative Name | OPN, SPP1, Secreted phosphoprotein 1, Bone sialoprotein 1 |
|---|---|
| Application | ELISA, IHC (FS), IHC (PS), WB |
| Clone | 87-B |
| Crossreactivity | Does not cross-react with mouse or bovine OPN. |
| Formulation | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
| Host | Mouse |
| Immunogen | Recombinant GST tagged human osteopontin. |
| Isotype | IgG2a |
| Purity Detail | Protein G affinity purified. |
| Recommendation Dilutions/Conditions | Immunohistochemistry: staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0, for 10min followed by cooling at RT for 20min.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Source | Purified from mouse ascites. |
| Species Reactivity | Human |
| Specificity | Recognizes full length OPN and C-terminal portion of thrombin-cleaved OPN. |
| Technical Info / Product Notes | For immunohistochemistry, staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH6.0 for 10min followed by cooling at RT for 20min. Recognizes full length human OPN and C-terminal portion of thrombin-cleaved human OPN. Does not react with mouse or bovine OPN. |
| UniProt ID | P10451 |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Handling | Avoid freeze/thaw cycles. |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |