Enzo ALX-201-073-C020 KillerTRAIL™ Protein (soluble) (human), (recombinant) (20 µg)

Apo-2L, TNFSF 10, CD253

Induces apoptosis in a concentration range of 10-100ng/ml.
Note: Does not require a cross-linking enhancer for its potent biological activity. For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SUPERKILLERTRAIL^® (Prod. No. ALX-201-115).

≤0.01 EU/μg purified protein (LAL test).

Liquid. HEPES, pH 7.4, sodium chloride, Tween 20, sucrose, azide free and 1mM DTT.

~24kDa (SDS-PAGE)

≥99% (SDS-PAGE, MS analysis)

Affinity purified. Sterile filtered.

For a special storage and dilution buffer see KillerTRAIL™ Storage and Dilution Buffer (Prod. No. ALX-505-005).

Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.

ED₅₀: 20ng/ml (BJAB cells)

Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG).

P50591

Stable for at least 12 months after receipt when stored at -80° C. 

Once thawed, prepare aliquots and store at -80°C (please note – to retain full activity the concentration of aliquots should be at least 0.1 mg/mL).  Avoid freeze/thaw cycles. Keep vial closely capped before use, spin the tube at 5000 x g for 30 sec before use.

-80°C

Dry Ice