Enzo ALX-201-115-C010 SUPERKILLERTRAIL® Protein (soluble) (human), (recombinant) (10 µg)

TNFSF 10, Apo-2L, CD253

Induces apoptosis at concentrations of >5ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).

0.5mg/ml

<0.01EU/µg purified protein (LAL test).

Liquid. HEPES, pH 7.4, sodium chloride, Tween 20, sucrose, azide free and 1mM DTT.

~26kDa

≥98% (SDS-PAGE). MS analysis, <1% impurity (mainly Hsp70 protein from E. coli)

Affinity purified. Sterile filtered.

Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Binds to human TRAIL receptors.

Click here for an example of SUPERKILLERTRAIL^® used by Eidgenössische Technische Hochschule (ETH) Zürich.

P50591

Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. In order to retain full activity aliquote concentration must be at least 0.1mg/ml; prepare 20µl aliquots and store at -80°C. Working dilution: 2-100ng/ml. Recommended: Prod. No. ALX-505-005, special storage and dilution buffer.

Avoid freeze/thaw cycles.

-80°C

Dry Ice