Enzo ALX-201-239-C010 Procathepsin K (human), (recombinant) (10 µg)

Procathepsin O, Procathepsin O2, Procathepsin X

150μg/ml

Liquid. In 25mM TRIS, pH 8.0, containing 500mM sodium chloride.

~35kDa

≥95% (SDS-PAGE)

Produced in E. coli (aa 19-329). Methionine was introduced at aa 18 to create a new N-terminal sequence (MEEIL).

≥1’000mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol of substrate per min. at 37°C, pH 5.5.

Inhibitors: Cathepsin K is inhibited by leupeptin (Prod. No. ALX-260-009) (IC₅₀=70nM), E-64 (IC₅₀=5nM) or cystatine. Minimal effects exhibit pepstatin, phenylmethylsulfonyl fluoride (Prod. No. ALX-270-184), inhibitors of aspartyl and serine proteases. No inhibition was observed by addition of EDTA or phenanthroline, classical inhibitors of metalloproteases.

P43235

Stable for several months when stored at -80°C. After activation, cathepsin is highly autoproteolytic. When not used immediately, add MMTS (Methyl methanethiosulfonate) to 1mM final concentration for stabilization and shock freeze in liquid nitrogen. Do not store dilutions of the enzyme.

Avoid freeze/thaw cycles.

-80°C

Dry Ice