Mfg Part Number
ALX-201-750-1

Enzo ALX-201-750-1 Hepsin (human), (recombinant) (125 U)

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Enzo ALX-201-750-1 Hepsin (human), (recombinant) (125 U)
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Alternative Name

Transmembrane protease serine 1, TMPRSS1

Biological Activity

Trypsin-like serine protease.

Concentration

100nM

Formulation

Liquid. In 100mM TRIS, pH 9.0, containing 200mM sodium chloride and 10% glycerol.

MW

~45kDa; a-subunit: ~15kDa; b-subunit: ~30kDa

Source

Produced in Drosophila Schneider 2 (S2) cells. Recombinant human hepsin (aa 45-417) is fused at the C-terminus to a V5-His-tag. Produced by plasmid transfection expression.

Specific Activity

~2.5U/µl. One unit is defined at the amount of enzyme that releases 1nmol of AMC per min. at 37°C in the presence of 50µM Boc-Gln-Ala-Arg-AMC.

UniProt ID

P05981

Use/Stability

Stable for at least 6 months when stored at -20°C. Recommended Assay Buffer: 100mM TRIS, pH 9.0, containing 5mg/ml BSA. Do not store diluted solutions.

Handling

Avoid freeze/thaw cycles.

Short Term Storage

-20°C

Long Term Storage

-80°C

Shipping

Dry Ice

More Information
Brand Enzo
UNSPSC 12352202