Mfg Part Number
ALX-201-853-0010

Enzo ALX-201-853-0010 eNOS (human), (recombinant) (10 µg)

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Enzo ALX-201-853-0010 eNOS (human), (recombinant) (10 µg)
$560.56
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Alternative Name

Nitric oxide synthase (endothelial), NOS III

Formulation

Liquid. In 50 mM TRIS, pH 7.4, containing 0.1mM EDTA, 10% glycerol, 100mM NaCl, 0.1mM DTT, 10 µM tetrahydrobiopterin.

MW

~135kDa/subunit; homodimer.

Purity

≥90% (SDS-PAGE, Western Blot)

Source

Produced in Sf9 insect cells. Untagged human eNOS. Produced in a baculovirus expression system.

Specific Activity

≥120 U/mg. 1 U is defined as the amount of enzyme required to produce 1 nmol of NO (nitric oxide) per minute at 37°C. NO synthesis is measured by the conversion of oxyhemoglobin to methemoglobin.

UniProt ID

P29474

Use/Stability

eNOS is relatively unstable, and loses activity on ice within 30 minutes. Prepare aliquots and snap-freeze in liquid nitrogen until ready for use.

Handling

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Long Term Storage

-80°C

Shipping

Dry Ice

More Information
Brand Enzo
UNSPSC 12352202