Enzo ALX-521-006-C010 Nerve growth factor 2.5S (mouse), (grade I) (10 µg)

NGF 2.5S

mNGF 2.5S was bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7 – 14 days. The neurotrophic activity is in the range of 0.5-50ng/ml. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50ng/ml medium.

93928-24-6

Lyophilized.

~26kDa; homodimer.

≥98% (HPLC)

mNGF 2.5S concentration is estimated spectroscopically from its extinction coefficient and amino acid analysis.

Reconstitute in sterile water or DMEM at a concentration of at least 10µg/ml and store at -80°C before use. Medium with mNGF cannot be reused due to mNGF degradation.

Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984).

P01139

As indicated on product label or CoA when stored as recommended. Stable for 2-3 weeks when stored at room temperature. After reconstitution, stable for up to 1 week when stored at +4°C and for 4-6 weeks at -80°C.

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

-20°C

Blue Ice