Enzo ALX-521-007-C100 Nerve growth factor 2.5S (mouse), (grade II) (100 µg)

NGF 2.5S

Neurotrophic activity was bioassayed using rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50ng/ml medium.

93928-24-6

Lyophilized.

~26kDa; homodimer.

≥95% (HPLC)

Concentration is estimated spectroscopically from its extinction coefficient and amino acid analysis.

Dilute in sterile water or DMEM to a concentration of at least 10µg/ml and store at -80°C before use.

Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984).

P01139

As indicated on product label or CoA when stored as recommended. Stable for 2-3 weeks when stored at room temperature. After reconstitution, stable for up to 1 week when stored at +4°C and for 4-6 weeks at -80°C.

Do not reuse medium containing mNGF. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

-20°C

Ambient Temperature