Enzo ALX-522-002-C050 Fas (human):Fc (human), (recombinant) (50 µg)

CD95, APO-1, TNFRSF 6

ELISA: binds to FasL at 10-100 ng/ml.

1mg/ml after reconstitution.

<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).

Lyophilized. Contains PBS.

~56 kDa

≥95% (SDS-PAGE)

Purified by multi-step chromatography.

Reconstitute with 50µl sterile water. Further dilutions can be made with medium containing 5% fetal calf serum or a carrier protein.

Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1) (aa 7-154) is fused to the Fc portion of human IgG1.

Binds human, mouse and rat FasL (APO-1L; CD95L; CD178).

Historical data has shown that Fas inhibits the activity of human and mouse soluble Fas (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells in a concentration range of 20-100µg/ml. When used with cross-linking enhancer (see Prod. No. ALX-203-001), the inhibitory activity of rhFas:Fc is increased approximately 20- to 50-fold. Concentrations of rhFas:Fc required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.

P25445

Stable for at least 6 months after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

-20°C

Blue Ice