Enzo ALX-522-008-C050 TNF-α (soluble) (human), (recombinant) (50 µg)

Tumor necrosis factor-α, TNFSF 2

ELISA: binds to TNF-R2 at 0.1-10 ng.

1mg/ml after reconstitution.

<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).

Lyophilized. Contains PBS.

~19kDa (SDS-PAGE).

≥95% (SDS-PAGE)

Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.

Binds to human and mouse TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (Prod. No. ALX-804-034), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.

Historical lots have demonstrated biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells), and an ED₅₀ of 0.1ng/ml (WEHI 164 cells).

FLAG is a registered trademark of Sigma-Aldrich Co.

P01375

Stable for at least 6 months after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

-20°C

Blue Ice