Enzo ALX-522-015-6010 CD40L (soluble) (human), (recombinant) (6 x 10 µg)

CD154, TNFSF 5, gp39

ELISA: binds to CD40 receptor at 1-10 ng/ml.

0.1mg/ml after reconstitution.

Lyophilized. Contains PBS.

~18kDa (SDS-PAGE).

≥95% (SDS-PAGE)

Purified by Multi-Step Chromatography.

Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG^®-tag.

Binds to human CD40.

Historical data has shown that CD40L stimulates growth of B cells. The activity of rhsCD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of cross-linking enhancer (see Set Prod. No. ALX-850-064). For stimulation of mouse cells via CD40 use CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070 or ALX-850-075).

FLAG is a registered trademark of Sigma-Aldrich Co.

P29965

Stable for at least 6 months after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

-20°C

Blue Ice