Mfg Part Number
ALX-522-016-C050

Enzo ALX-522-016-C050 CD40 (human):Fc (human), (recombinant) (50 µg)

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Enzo ALX-522-016-C050 CD40 (human):Fc (human), (recombinant) (50 µg)
$535.60
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Alternative Name

TNFRSF 5

Application Notes

ELISA: binds to CD40L at 1-10 ng/ml.
Can be used together with anti-human IgG1 PAb to detect membrane-bound human or mouse CD40L. CD40:Fc should be used at concentrations between 1 and 100µg/ml depending on the level of CD40L surface expression.

Concentration

1mg/ml after reconstitution.

Endotoxin Content

<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc).

Formulation

Lyophilized. Contains PBS.

MW

~54kDa (SDS-PAGE).

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Source

Produced in HEK 293 cells. The cysteine-rich region (aa 21-193) of human CD40 is fused to the Fc portion of human IgG1.

Specificity

Binds human and mouse CD40L (CD154).

UniProt ID

P25942

Use/Stability

Stable for at least 6 months after receipt when stored at -20°C.

Handling

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Long Term Storage

-20°C

Shipping

Blue Ice

More Information
Brand Enzo
UNSPSC 12352202