Enzo ALX-522-020-C005 SUPERFASLIGAND® Protein (soluble) (human), (recombinant) (5 µg)

FasL (oligomer), APO-1L (oligomer), CD95L (oligomer), CD178 (oligomer), TNFSF 6 (oligomer)

ELISA: binds to Fas receptor at 1-100 ng/ml.

Kills Fas-sensitive cells. Note: Does not require enhancer.

0.1mg/ml after reconstitution.

Lyophilized. Contains PBS.

~32kDa (nonglycosylated), ~35kDa (glycosylated).

≥95% (SDS-PAGE)

Reconstitute with 50µl sterile water. Further dilutions should be made with cell culture medium containing 5% fetal calf serum.

Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG^®-tag. Glycosylation of rhs SUPERFASLIGAND^® is similar to natural human FasL.

Binds to human, mouse and rat Fas (CD95; APO-1).

Historical data has shown that SUPERFASLIGAND^® kills Fas-sensitive cells at concentrations of >1ng/ml without the use of enhancer. The ED₅₀ has been shown in previous data to be 1ng/ml (A20 cells).

Note: Results using rhsSUPERFASLIGAND^® may differ from those obtained with agonistic antibodies!

FLAG is a registered trademark of Sigma-Aldrich Co.

P48023

Stable for at least 6 months after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

-20°C

Blue Ice