Enzo ALX-522-120-C010 MEGACD40L® Protein (soluble) (mouse), (recombinant) (10 µg)

CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF5:ACRP30headless, gp39:ACRP30headless

Binds to mouse and human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).

0.1mg/ml after reconstitution.

<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.).

Lyophilized. Contains PBS.

~35-40kDa

≥95% (SDS-PAGE)

Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Produced in CHO cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG^®-tag.

FLAG is a registered trademark of Sigma-Aldrich Co.

P27548 (CD40L), Q60994 (ACRP30)

Stable for at least 6 months after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

-20°C

Blue Ice