Enzo ALX-522-124-C010 Netrin-1 (mouse), (recombinant) (10 µg)

ELISA: binds to UNC5B.

0.1mg/ml after reconstitution.

<0.1EU/µg purified protein (LAL test; Bio Whittaker).

Lyophilized. Contains PBS.

~80kDa

≥90% (SDS-PAGE)

Purified by multi-step chromatography.

Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.

Produced in HEK 293 cells. The extracellular domain of mouse netrin-1 (aa 27-604) is fused at the C-terminus to a FLAG^®-tag.

Binds to human and mouse UNC5B.

FLAG is a registered trademark of Sigma-Aldrich Co.

O09118

Stable for at least 6 months after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

-20°C

Blue Ice