Enzo ALX-801-008-C150 P-glycoprotein (human) monoclonal antibody (MRK16) (150 µg)
| Alternative Name | ABCB1, CD243, P-glycoprotein 1 |
|---|---|
| Application | Flow Cytometry, FUNC, IHC (FS), IP |
| Application Notes | Functional Application: Inhibits P-glycoprotein function. |
| Clone | MRK16 |
| Crossreactivity | Does not cross-react with mouse P-glycoprotein. |
| Formulation | Liquid. In PBS containing 1% BSA and 0.1% sodium azide. |
| Host | Mouse |
| Immunogen | Adriamycin-resistant human myelogenous leukemia K-562/ADM cells. |
| Isotype | IgG2a |
| Purity Detail | Protein A affinity purified. |
| Recommendation Dilutions/Conditions | Flow Cytometry: For 5×105 pelleted cells use 50µl of 10-100µg/ml.Immunohistochemistry (frozen sections): 0.1-10µg/ml. Use mild fixation treatment such as 4% paraformaldehyde. Do not use acetone, paraffin, methanol or ethanol as a fixative. Iodine labelling can also be performed on MRK16.Immunoprecipitation: 25-50µg/mlSuggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Species Reactivity | Human |
| Specificity | Recognizes an external epitope of human P-glycoprotein. Also recognizes P-glycoprotein of human cortical adenomas. |
| UniProt ID | P08183 |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Handling | Avoid freeze/thaw cycles. |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |