Enzo ALX-803-080-C100 LC3B monoclonal antibody (5F10) (100 µg)

Microtubule-associated protein 1 light chain 3B, MAP1LC3B

ICC, WB

Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.

5F10

Lyophilized from 1ml PBS containing PEG, sucrose, and 0.09% sodium azide.

Mouse

Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.

IgG1

Included. (Prod. No. ALX-840-038)

Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.

Immunocytochemistry (paraformaldehyde/methanol fixed cells; 1-10 µg/ml)Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)We strongly recommend to use PVDF membranes for immunoblot analysis.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitute with 1ml water (15 minutes at room temperature).

Dog, Hamster, Human, Mouse, Rat

Recognizes both forms of endogenous LC3B.

Q9GZQ8

This antibody is covered by our Worry-Free Guarantee.

Stable at -80°C up to 1 year, at 4°C up to 3 months.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.

-20°C

Blue Ice