Enzo ALX-803-082-C100 LC3 monoclonal antibody (5H3) (100 µg)
| Alternative Name | Microtubule-associated protein 1 light chain 3, MAP1LC3 |
|---|---|
| Application | ICC, IHC, WB |
| Application Notes | Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot. |
| Clone | 5H3 |
| Formulation | Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose. |
| Host | Mouse |
| Immunogen | Synthetic peptide corresponding to an internal sequence identical in LC3A, LC3B and LC3C conjugated to hemocyanin. |
| Isotype | IgG1 |
| Positive Control | Included. (Prod. No. ALX-840-038) |
| Purity Detail | Thiophilic adsorption and size exclusion chromatography purified. |
| Recommendation Dilutions/Conditions | Immunocytochemistry (paraformaldehyde/methanol fixation; 1-10 µg/ml)Western Blot (0.5µg/ml for HRPO/ECL detection; Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)We strongly recommend to use PVDF membranes for immunoblot assays.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Species Reactivity | Human |
| Specificity | Recognizes both forms of endogenous LC3. |
| UniProt ID | Q9H492 (LC3A), Q9GZQ8 (LC3B), Q9BXW4 (LC3C) |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Use/Stability | Stable at -20°C up to 1 year. |
| Handling | Thawed aliquots may be stored at 4°C up to 3 months. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |