Enzo ALX-804-061-C100 ERK1/2 monoclonal antibody (12D4) (100 µg)

MAPK2/3, MAP kinase 2/3

ELISA, ICC, IHC, IP, WB

12D4

Does not cross-react with activated JNK or p38.

Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Mouse

Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2 conjugated to KLH.

IgG1

Included. (Prod. No. ALX-840-025)

Thiophilic adsorption and size exclusion chromatography purified.

Immunocytochemistry (1-10µg/ml)Immunoprecipitation (1-10µg per 106 pervanadate-treated A431 or HepG2 cells)Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitute with 1ml water (15 minutes at room temperature).

Dog, Human, Mouse, Rat

Recognizes the pThr-Glu-pTyr motif of activated ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein.

Replacement for ADI-905-651

P27361 (Erk1), P28482 (Erk2)

This antibody is covered by our Worry-Free Guarantee.

Stable at -80°C up to 1 year, at 4°C up to 3 months.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.

-20°C

Blue Ice