Enzo ALX-804-064-C100 EGF receptor (human) monoclonal antibody (13G8) (100 µg)
| Alternative Name | Epidermal growth factor receptor, EGFR, HER-1 |
|---|---|
| Application | ELISA, ICC, IP, Luminex, WB |
| Clone | 13G8 |
| Formulation | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Host | Mouse |
| Immunogen | Synthetic peptide corresponding to aa 1165-1186 of EGF receptor (epidermal growth factor receptor) conjugated to KLH. |
| Isotype | IgG1 |
| Positive Control | Included. (Prod. No. ALX-840-030) |
| Purity Detail | Thiophilic adsorption and size exclusion chromatography purified. |
| Recommendation Dilutions/Conditions | Immunocytochemistry (0.1-1µg/ml)Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Reconstitution | Reconstitute with 1ml water (15 minutes at room temperature). |
| Species Reactivity | Human, Mouse |
| Specificity | Recognizes the C-terminus of the EGF receptor (aa 1165-1186). Recognition is independent of phosphorylation of Tyr1173. |
| UniProt ID | P00533 |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Use/Stability | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
| Handling | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |