Enzo ALX-804-101-C100 α-Catenin monoclonal antibody (15D9) (100 µg)
| Application | ICC, IP, WB |
|---|---|
| Clone | 15D9 |
| Crossreactivity | Does not cross-react with β-catenins. |
| Formulation | Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose. |
| Host | Mouse |
| Immunogen | Recombinant human α-catenin. |
| Isotype | IgG1 |
| Positive Control | Included. (Prod. No. ALX-840-026) |
| Purity Detail | Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. |
| Recommendation Dilutions/Conditions | Immunocytochemistry (0.1-1µg/ml; formaldehyde fixation is tolerated)Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Reconstitution | Reconstitute with 1ml distilled water (15 min., RT). |
| Species Reactivity | Dog, Human, Mouse, Rat |
| UniProt ID | P35221 (α1), P26232 (α2), Q9UI47 (α3) |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Use/Stability | Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months. |
| Handling | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |