Enzo ALX-804-351-C100 BimS/EL/L monoclonal antibody (14A8) (100 µg)

BCL2L11

Flow Cytometry, ICC, IHC (PS), IP, WB

14A8

Liquid in PBS containing 0.02% sodium azide.

Rat

Recombinant mouse BimS.

IgG2a

Protein G-affinity purified.

Flow Cytometry (0.5-1µg/ml; overexpressing cell lines)Immunocytochemistry (1-5µg/ml)Immunohistochemistry (paraffin sections, 1-5µg/ml)Immunoprecipitation (1-2µg/ml)Western Blot (1-2µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Human, Monkey, Mouse

Recognizes Bim_L, Bim_EL and Bim_S.

As Bim expression is generally low in non-transfected cell lines, please take care when choosing positive controls. Examples of cell lines which express detectable levels of Bim include: B6.2.16.BW2 or KO52.DA20 (T cell lymphomas) or ABL5.8.1 (B cell lymphoma) or MCF-7s. It is generally necessary to use a high amount of lysate (e.g. 1 x 10^7 cells). When using tissue samples, it is generally necessary to use 1-5mg of total protein from an appropriate tissue source.

O54918

This antibody is covered by our Worry-Free Guarantee.

Avoid freeze/thaw cycles.

+4°C

-20°C

Blue Ice