Enzo ALX-804-358-C100 [pSer36]Shc monoclonal antibody (6E10) (100 µg)
| Alternative Name | Shc transforming protein 1, SH2 domain protein C1, Shc1 |
|---|---|
| Application | WB |
| Clone | 6E10 |
| Crossreactivity | Does not cross-react with non-phosphorylated shc. |
| Formulation | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Host | Mouse |
| Immunogen | Synthetic peptide corresponding to aa 33-39 (E33LPpSPSA39) of shc phosphorylated at Ser36 conjugated to KLH. |
| Isotype | IgG1 |
| Positive Control | Included. (Prod. No. 840-033-1) |
| Purity Detail | Thiophilic adsorption and size exclusion chromatography purified. |
| Recommendation Dilutions/Conditions | Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 1% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 2 x PBS). Immunoprecipitation of shc prior to detection with MAb to Shc (phosphorylated) (pSer36) (6E10) is recommendedSuggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Reconstitution | Reconstitute with 1ml water (15 minutes at room temperature). |
| Species Reactivity | Dog, Human, Mouse |
| UniProt ID | P29353 |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Use/Stability | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
| Handling | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |