Enzo ALX-804-457-C125 Proteinase inhibitor 9 (human) monoclonal antibody (PI9-17) (125 µg)
| Alternative Name | PI9, Serpin B9, CAP3, Cytoplasmic antiproteinase 3 |
|---|---|
| Application | IHC (PS), WB |
| Application Notes | Detects bands of ~42kDa (native PI9) and ~67kDa (PI9 complexed to granzyme B) by Western blot. |
| Clone | PI9-17 |
| Crossreactivity | Does not cross-react with PI6, PI8 or PAI-2. |
| Formulation | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
| Host | Mouse |
| Immunogen | Recombinant human full-length PI9 (proteinase inhibitor 9). |
| Isotype | IgG1 |
| Positive Control | YT-Indy cells (a natural killer leukemia cell line) can be used as positive control. In other cell lines a non-specific protein band of ~63kDa can be detected. |
| Recommendation Dilutions/Conditions | Immunohistochemistry (formaldehyde-fixed paraffin-embedded human tissues after pretreatment with 10mM citric acid pH 6.0 for 20min; use ~1:100. For fixed cytospin preparations fix cells for 10min in 10% formalin and then treat them as paraffin sections)Western Blot (standard procedures)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Species Reactivity | Human |
| UniProt ID | P50453 |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |